Positive residues involved in the voltage-gating of the mitochondrial porin-channel are localized in the external moiety of the pore
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Positive residues involved in the voltage-gating of the mitochondrial porin-channel are localized in the external moiety of the pore.
The role of positive charges located on the hydrophilic surface of the mitochondrial outer membrane channel was investigated by studying the interaction between LDAO-solubilized porin and a cation-exchanger column. The binding of porin to the column material was inhibited when the elution buffer had a pH of 9 or when 2 mM dextran sulfate was added to the buffer at neutral pH. Interestingly, the...
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